diff --git a/buildroot/share/PlatformIO/scripts/stm32_serialbuffer.py b/buildroot/share/PlatformIO/scripts/stm32_serialbuffer.py
index fecce47db3..c3779289e0 100644
--- a/buildroot/share/PlatformIO/scripts/stm32_serialbuffer.py
+++ b/buildroot/share/PlatformIO/scripts/stm32_serialbuffer.py
@@ -3,26 +3,57 @@
 #
 Import("env")
 
-# Marlin has `RX_BUFFER_SIZE` and `TX_BUFFER_SIZE` to configure the
-# buffer size for receiving and transmitting data respectively.
-# Stm32duino uses another set of defines for the same purpose,
-# so we get the values from the Marlin configuration and set
-# them in `SERIAL_RX_BUFFER_SIZE` and `SERIAL_TX_BUFFER_SIZE`.
-# It is not possible to change the values at runtime, they must
-# be set with build flags.
+# Marlin uses the `RX_BUFFER_SIZE` \ `TX_BUFFER_SIZE` options to
+# configure buffer sizes for receiving \ transmitting serial data.
+# Stm32duino uses another set of defines for the same purpose, so this
+# script gets the values from the configuration and uses them to define
+# `SERIAL_RX_BUFFER_SIZE` and `SERIAL_TX_BUFFER_SIZE` as global build
+# flags so they are available for use by the platform.
 #
 # The script will set the value as the default one (64 bytes)
 # or the user-configured one, whichever is higher.
 #
-# Marlin has 128 and 32 as default values for RX_BUFFER_SIZE and
-# TX_BUFFER_SIZE respectively. We use the highest value.
+# Marlin's default buffer sizes are 128 for RX and 32 for TX.
+# The highest value is taken (128/64).
+#
+# If MF_*_BUFFER_SIZE, SERIAL_*_BUFFER_SIZE, USART_*_BUF_SIZE, are
+# defined, the first of these values will be used as the minimum.
+build_flags = env.ParseFlags(env.get('BUILD_FLAGS'))["CPPDEFINES"]
 mf = env["MARLIN_FEATURES"]
-rxBuf = str(max(128, int(mf["RX_BUFFER_SIZE"]) if "RX_BUFFER_SIZE" in mf else 0))
-txBuf = str(max(64, int(mf["TX_BUFFER_SIZE"]) if "TX_BUFFER_SIZE" in mf else 0))
 
-build_flags = env.get('BUILD_FLAGS')
-build_flags.append("-DSERIAL_RX_BUFFER_SIZE=" + rxBuf)
-build_flags.append("-DSERIAL_TX_BUFFER_SIZE=" + txBuf)
-build_flags.append("-DUSART_RX_BUF_SIZE=" + rxBuf)
-build_flags.append("-DUSART_TX_BUF_SIZE=" + txBuf)
-env.Replace(BUILD_FLAGS=build_flags)
+# Get a build flag's value or None
+def getBuildFlagValue(name):
+	for flag in build_flags:
+		if isinstance(flag, list) and flag[0] == name:
+			return flag[1]
+
+	return None
+
+# Get an overriding buffer size for RX or TX from the build flags
+def getInternalSize(side):
+	return	getBuildFlagValue(f"MF_{side}_BUFFER_SIZE") or \
+			getBuildFlagValue(f"SERIAL_{side}_BUFFER_SIZE") or \
+			getBuildFlagValue(f"USART_{side}_BUF_SIZE")
+
+# Get the largest defined buffer size for RX or TX
+def getBufferSize(side, default):
+	# Get a build flag value or fall back to the given default
+	internal = int(getInternalSize(side) or default)
+	flag = side + "_BUFFER_SIZE"
+	# Return the largest value
+	return max(int(mf[flag]), internal) if flag in mf else internal
+
+# Add a build flag if it's not already defined
+def tryAddFlag(name, value):
+	if getBuildFlagValue(name) is None:
+		env.Append(BUILD_FLAGS=[f"-D{name}={value}"])
+
+# Get the largest defined buffer sizes for RX or TX, using defaults for undefined
+rxBuf = getBufferSize("RX", 128)
+txBuf = getBufferSize("TX",  64)
+
+# Provide serial buffer sizes to the stm32duino platform
+tryAddFlag("SERIAL_RX_BUFFER_SIZE", rxBuf)
+tryAddFlag("SERIAL_TX_BUFFER_SIZE", txBuf)
+tryAddFlag("USART_RX_BUF_SIZE", rxBuf)
+tryAddFlag("USART_TX_BUF_SIZE", txBuf)
diff --git a/ini/stm32f4.ini b/ini/stm32f4.ini
index 901f145113..f77f03e375 100644
--- a/ini/stm32f4.ini
+++ b/ini/stm32f4.ini
@@ -154,8 +154,8 @@ board_build.variant = MARLIN_BIGTREE_E3_RRF
 board_build.offset  = 0x8000
 build_flags         = ${stm32_variant.build_flags}
                       -DSTM32F407_5VX
-                      -DSERIAL_RX_BUFFER_SIZE=255
-                      -DSERIAL_TX_BUFFER_SIZE=255
+                      -DMF_RX_BUFFER_SIZE=255
+                      -DMF_TX_BUFFER_SIZE=255
 
 #
 # Bigtreetech GTR V1.0 (STM32F407IGT6 ARM Cortex-M4)